affigel blue column Search Results


deae affigel blue column  (Bio-Rad)
93
Bio-Rad deae affigel blue column
Deae Affigel Blue Column, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
deae affigel blue column - by Bioz Stars, 2026-04
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affigel blue column  (Bio-Rad)
96
Bio-Rad affigel blue column
Affigel Blue Column, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/affigel blue column/product/Bio-Rad
Average 96 stars, based on 1 article reviews
affigel blue column - by Bioz Stars, 2026-04
96/100 stars
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affigel blue column  (Millipore)
90
Millipore affigel blue column
(A) Denaturing gel analysis of the purified PAP fractions from <t>Affigel</t> Blue column by SDS-PAGE and stained by silver nitrate. Lanes 1 and 11, Bio-Rad Precision plus protein standards; lanes 2–4, 0.28, 0.55 and 0.83 µg of fraction #4; lanes 5–7, 0.19, 0.37 and 0.56 µg of fraction #5; lanes 8–10, 0.30, 0.60 and 0.90 µg of the pooled fractions 11–17. (B) PAP activity determined by in-gel phosphatase assay with DiFMUP. Lanes 1 and 8, positive control B-phycoerythrin; lanes 2–7, 0.5, 1, 3, 6, 10 and 12 µg of PAP from the pooled fraction. (C) Native gel analysis of the purified PAP fractions stained with Coomassie Blue. Lanes 1 and 8, native marker unstained protein standards; lanes 2–7, 0.5, 1, 3, 6, 10 and 12 µg of PAP from the pooled fraction.
Affigel Blue Column, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/affigel blue column/product/Millipore
Average 90 stars, based on 1 article reviews
affigel blue column - by Bioz Stars, 2026-04
90/100 stars
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cibacron blue agarose column  (Bio-Rad)
90
Bio-Rad cibacron blue agarose column
(A) Denaturing gel analysis of the purified PAP fractions from <t>Affigel</t> Blue column by SDS-PAGE and stained by silver nitrate. Lanes 1 and 11, Bio-Rad Precision plus protein standards; lanes 2–4, 0.28, 0.55 and 0.83 µg of fraction #4; lanes 5–7, 0.19, 0.37 and 0.56 µg of fraction #5; lanes 8–10, 0.30, 0.60 and 0.90 µg of the pooled fractions 11–17. (B) PAP activity determined by in-gel phosphatase assay with DiFMUP. Lanes 1 and 8, positive control B-phycoerythrin; lanes 2–7, 0.5, 1, 3, 6, 10 and 12 µg of PAP from the pooled fraction. (C) Native gel analysis of the purified PAP fractions stained with Coomassie Blue. Lanes 1 and 8, native marker unstained protein standards; lanes 2–7, 0.5, 1, 3, 6, 10 and 12 µg of PAP from the pooled fraction.
Cibacron Blue Agarose Column, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
cibacron blue agarose column - by Bioz Stars, 2026-04
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deae-affigel blue column  (Bio-Rad)
90
Bio-Rad deae-affigel blue column
(A) Denaturing gel analysis of the purified PAP fractions from <t>Affigel</t> Blue column by SDS-PAGE and stained by silver nitrate. Lanes 1 and 11, Bio-Rad Precision plus protein standards; lanes 2–4, 0.28, 0.55 and 0.83 µg of fraction #4; lanes 5–7, 0.19, 0.37 and 0.56 µg of fraction #5; lanes 8–10, 0.30, 0.60 and 0.90 µg of the pooled fractions 11–17. (B) PAP activity determined by in-gel phosphatase assay with DiFMUP. Lanes 1 and 8, positive control B-phycoerythrin; lanes 2–7, 0.5, 1, 3, 6, 10 and 12 µg of PAP from the pooled fraction. (C) Native gel analysis of the purified PAP fractions stained with Coomassie Blue. Lanes 1 and 8, native marker unstained protein standards; lanes 2–7, 0.5, 1, 3, 6, 10 and 12 µg of PAP from the pooled fraction.
Deae Affigel Blue Column, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/deae-affigel blue column/product/Bio-Rad
Average 90 stars, based on 1 article reviews
deae-affigel blue column - by Bioz Stars, 2026-04
90/100 stars
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protein a-sepharose (affigel blue column  (Bio-Rad)
90
Bio-Rad protein a-sepharose (affigel blue column
(A) Denaturing gel analysis of the purified PAP fractions from <t>Affigel</t> Blue column by SDS-PAGE and stained by silver nitrate. Lanes 1 and 11, Bio-Rad Precision plus protein standards; lanes 2–4, 0.28, 0.55 and 0.83 µg of fraction #4; lanes 5–7, 0.19, 0.37 and 0.56 µg of fraction #5; lanes 8–10, 0.30, 0.60 and 0.90 µg of the pooled fractions 11–17. (B) PAP activity determined by in-gel phosphatase assay with DiFMUP. Lanes 1 and 8, positive control B-phycoerythrin; lanes 2–7, 0.5, 1, 3, 6, 10 and 12 µg of PAP from the pooled fraction. (C) Native gel analysis of the purified PAP fractions stained with Coomassie Blue. Lanes 1 and 8, native marker unstained protein standards; lanes 2–7, 0.5, 1, 3, 6, 10 and 12 µg of PAP from the pooled fraction.
Protein A Sepharose (Affigel Blue Column, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
protein a-sepharose (affigel blue column - by Bioz Stars, 2026-04
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aurum affigel blue columns  (Bio-Rad)
96
Bio-Rad aurum affigel blue columns
(A) Denaturing gel analysis of the purified PAP fractions from <t>Affigel</t> Blue column by SDS-PAGE and stained by silver nitrate. Lanes 1 and 11, Bio-Rad Precision plus protein standards; lanes 2–4, 0.28, 0.55 and 0.83 µg of fraction #4; lanes 5–7, 0.19, 0.37 and 0.56 µg of fraction #5; lanes 8–10, 0.30, 0.60 and 0.90 µg of the pooled fractions 11–17. (B) PAP activity determined by in-gel phosphatase assay with DiFMUP. Lanes 1 and 8, positive control B-phycoerythrin; lanes 2–7, 0.5, 1, 3, 6, 10 and 12 µg of PAP from the pooled fraction. (C) Native gel analysis of the purified PAP fractions stained with Coomassie Blue. Lanes 1 and 8, native marker unstained protein standards; lanes 2–7, 0.5, 1, 3, 6, 10 and 12 µg of PAP from the pooled fraction.
Aurum Affigel Blue Columns, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
aurum affigel blue columns - by Bioz Stars, 2026-04
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affigel-blue column chromatography  (Bio-Rad)
90
Bio-Rad affigel-blue column chromatography
(A) Denaturing gel analysis of the purified PAP fractions from <t>Affigel</t> Blue column by SDS-PAGE and stained by silver nitrate. Lanes 1 and 11, Bio-Rad Precision plus protein standards; lanes 2–4, 0.28, 0.55 and 0.83 µg of fraction #4; lanes 5–7, 0.19, 0.37 and 0.56 µg of fraction #5; lanes 8–10, 0.30, 0.60 and 0.90 µg of the pooled fractions 11–17. (B) PAP activity determined by in-gel phosphatase assay with DiFMUP. Lanes 1 and 8, positive control B-phycoerythrin; lanes 2–7, 0.5, 1, 3, 6, 10 and 12 µg of PAP from the pooled fraction. (C) Native gel analysis of the purified PAP fractions stained with Coomassie Blue. Lanes 1 and 8, native marker unstained protein standards; lanes 2–7, 0.5, 1, 3, 6, 10 and 12 µg of PAP from the pooled fraction.
Affigel Blue Column Chromatography, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/affigel-blue column chromatography/product/Bio-Rad
Average 90 stars, based on 1 article reviews
affigel-blue column chromatography - by Bioz Stars, 2026-04
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cm22 affigel blue column  (Bio-Rad)
90
Bio-Rad cm22 affigel blue column
(A) Denaturing gel analysis of the purified PAP fractions from <t>Affigel</t> Blue column by SDS-PAGE and stained by silver nitrate. Lanes 1 and 11, Bio-Rad Precision plus protein standards; lanes 2–4, 0.28, 0.55 and 0.83 µg of fraction #4; lanes 5–7, 0.19, 0.37 and 0.56 µg of fraction #5; lanes 8–10, 0.30, 0.60 and 0.90 µg of the pooled fractions 11–17. (B) PAP activity determined by in-gel phosphatase assay with DiFMUP. Lanes 1 and 8, positive control B-phycoerythrin; lanes 2–7, 0.5, 1, 3, 6, 10 and 12 µg of PAP from the pooled fraction. (C) Native gel analysis of the purified PAP fractions stained with Coomassie Blue. Lanes 1 and 8, native marker unstained protein standards; lanes 2–7, 0.5, 1, 3, 6, 10 and 12 µg of PAP from the pooled fraction.
Cm22 Affigel Blue Column, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cm22 affigel blue column/product/Bio-Rad
Average 90 stars, based on 1 article reviews
cm22 affigel blue column - by Bioz Stars, 2026-04
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Image Search Results


(A) Denaturing gel analysis of the purified PAP fractions from Affigel Blue column by SDS-PAGE and stained by silver nitrate. Lanes 1 and 11, Bio-Rad Precision plus protein standards; lanes 2–4, 0.28, 0.55 and 0.83 µg of fraction #4; lanes 5–7, 0.19, 0.37 and 0.56 µg of fraction #5; lanes 8–10, 0.30, 0.60 and 0.90 µg of the pooled fractions 11–17. (B) PAP activity determined by in-gel phosphatase assay with DiFMUP. Lanes 1 and 8, positive control B-phycoerythrin; lanes 2–7, 0.5, 1, 3, 6, 10 and 12 µg of PAP from the pooled fraction. (C) Native gel analysis of the purified PAP fractions stained with Coomassie Blue. Lanes 1 and 8, native marker unstained protein standards; lanes 2–7, 0.5, 1, 3, 6, 10 and 12 µg of PAP from the pooled fraction.

Journal: PLoS ONE

Article Title: Characterization of a Soluble Phosphatidic Acid Phosphatase in Bitter Melon ( Momordica charantia )

doi: 10.1371/journal.pone.0106403

Figure Lengend Snippet: (A) Denaturing gel analysis of the purified PAP fractions from Affigel Blue column by SDS-PAGE and stained by silver nitrate. Lanes 1 and 11, Bio-Rad Precision plus protein standards; lanes 2–4, 0.28, 0.55 and 0.83 µg of fraction #4; lanes 5–7, 0.19, 0.37 and 0.56 µg of fraction #5; lanes 8–10, 0.30, 0.60 and 0.90 µg of the pooled fractions 11–17. (B) PAP activity determined by in-gel phosphatase assay with DiFMUP. Lanes 1 and 8, positive control B-phycoerythrin; lanes 2–7, 0.5, 1, 3, 6, 10 and 12 µg of PAP from the pooled fraction. (C) Native gel analysis of the purified PAP fractions stained with Coomassie Blue. Lanes 1 and 8, native marker unstained protein standards; lanes 2–7, 0.5, 1, 3, 6, 10 and 12 µg of PAP from the pooled fraction.

Article Snippet: Enzyme fraction from Affigel Blue column was concentrated in a Centricon concentrator (Amicon, centrifugal filter devices, Millipore Corporation) and during the course the imidazole buffer was exchanged to protease digestion buffer (50 mM ammonium carbonate, pH 8.0).

Techniques: Purification, SDS Page, Staining, Activity Assay, Phosphatase Assay, Positive Control, Marker